Regulatory

Part:BBa_J100036:Design

Designed by: Erich Baker   Group: Campbell M Lab   (2011-09-01)

Promoter induced by DNA damage


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Entered DNA sequence into this tool, http://gcat.davidson.edu/iGem10/index.html, which revealed overlapping oligonucleotide sequences.

The picture below shows how we got the oligos to build this promoter.

PolB.png


Source

E. coli, polB gene

References

Lewis, L, Jenkins, M, Mount, D. 1992. Isolation of DNA Damage-Inducible Promoters in Escherichia coli: Regulation of polB (dinA), dinG, and dinH by LexA Repressor. Journal of Bacteriology. 174. 10:3377-3385